“I need clarification. Can either m.2 slot be the boot slot? Also, do any of the sata ports become disabled with the use of the m.2 slots?”
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Yes, either M.2 slot can house the boot drive. The SATA ports will all remain active regardless of configuration. https://www.asus.com/us/Motherboards/TUF-GAMING-X570-PLUS-WI-FI/specifications/
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