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Yes, both use the RF lens mount. But bear in mind this lens is designed for full-frame sensor size cameras, whereas the R100 has an APS-C sensor. So it would behoove you to understand the consequences. Among other things, of course the effective focal length range will be 38.4 - 384 mm (assuming the crop factor of the R100 is 1.6, and I base that assumption on the fact that was the case for Canon's M series of APS-C cameras).
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