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When the M.2_2 is occupied by M.2 device, PCIe x16_1 will run at x8 mode. https://www.asus.com/us/Motherboards/ROG-STRIX-B450-F-GAMING/specifications/
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When the M.2_2 is occupied by M.2 device, PCIe x16_1 will run at x8 mode. https://www.asus.com/us/Motherboards/ROG-STRIX-B450-F-GAMING/specifications/