A:Answer Only the 2 PCI-e x1 slots will be available for use as the PCI-e x16 slot will be used by the GeForce GTX 1060 graphics card. Please feel free to call us if you have any other questions at 1.800.707.0393. In general wireless network cards will always be PCI-e x1 otherwise can be used in any PCI-e slot available (x1, x4, x16). Please feel free to call us if you have any other questions at 1.800.707.0393.